Server Application Unavailable.

I am using Windows 2003 Server Standard edition, i just prepared this
machine to use for one ASP.net application. i am using IIS 6.
ASP.net application runs fine but it become unavailable in every hour with a
big red color message "Server Application Unavailable".

I did search on net and did set machine.config to use "System" as user and
restarted machine. but it did not worked. i am unable to fix tihs error. any
idea what else i can change or verify to fix this error??? this error is
coming only in ASP.net application. i am also using simple ASP pages but
they are running fine but ASP.net application give this error. Please Help


.

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